HspA9 (11118P-10)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 10ug | WB | Human |  |
Overview
Product Name HspA9 (11118P-10)
Description Recombinant human HspA9 produced in E. coli is a single, non-glycosylated polypeptide containing 654 amino acidsaa 47-679) with a molecular weight of 71kDa. It is expressed with a 20-amino acid His-tag at the N-terminus.
Target HspA9
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution in 20mM Tris-Hcl, pH 8.0, 0.5mM DTT and 10% glycerol.
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 10% glycerol
Purity Greater than 95% as determined by SDS-PAGE
Background HspA9, a memer of the Hsp70 family, encodes a heat-shock cognate protein that is involved in the control of cell proliferation and acts as a chaperone. HspA9 is located on chromosome 5, band q31, a region thatis often deleted in myeloid leukemias and myelodysplasia, making it a candidate tumor suppressor gene. HspA9 suppresses nuclear translocation, transcriptional activation, and control centrosome-duplication functions of p53.
Specificity Information
Target Name Stress-70 protein, mitochondrial
Target ID HspA9
Alternative Names Hsp70 protein 9
Sequence Location Mitochondrion , Nucleus, nucleolus
Sequence GSSHHHHHH SSGLVPRGSH MASEAIKGAV VGIDLGTTNS CVAVMEGKQA KVLENAEGAR TTPSVVAFTA DGERLVGMPA KRQAVTNPNN TFYATKRLIG RRYDDPEVQK DIKNVPFKIV RASNGDAWVE AHGKLYSPSQ IGAFVLMKMK ETAENYLGHT AKNAVITVPA YFNDSQRQAT KDAGQISGLN VLRVINEPTA AALAYGLDKS EDKVIAVYDL GGGTFDISIL EIQKGVFEVK STNGDTFLGG EDFDQALLRH IVKEFKRETG VDLTKDNMAL QRVREAAEKA KCELSSSVQT DINLPYLTMD SSGPKHLNMK LTRAQFEGIV TDLIRRTIAP CQKAMQDAEV SKSDIGEVIL VGGMTRMPKV QQTVQDLFGR APSKAVNPDE AVAIGAAIQG GVLAGDVTDV LLLDVTPLSL GIETLGGVFT KLINRNTTIP TKKSQVFSTA ADGQTQVEIK VCQGEREMAG DNKLLGQFTL IGIPPAPRGV PQIEVTFDID ANGIVHVSAK DKGTGREQQI VIQSSGGLSK DDIENMVKNA EKYAEEDRRK KERVEAVNMA EGIIHDTETK MEEFKDQLPA DECNKLKEEI SKMRELLARK DSETGENIRQ AASSLQQASL KLFEMAYKKM ASEREGSGSS GTGEQKEDQK EEKQ.
Biological Function Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (PubMed:26702583). Regulates erythropoiesis via stabilization of ISC assembly (PubMed:21123823, PubMed:26702583). May play a role in the control of cell proliferation and cellular aging (By similarity). {UniProtKB:P38647, PubMed:21123823, PubMed:26702583}.
Background HspA9, a memer of the Hsp70 family, encodes a heat-shock cognate protein that is involved in the control of cell proliferation and acts as a chaperone. HspA9 is located on chromosome 5, band q31, a region thatis often deleted in myeloid leukemias and myelodysplasia, making it a candidate tumor suppressor gene. HspA9 suppresses nuclear translocation, transcriptional activation, and control centrosome-duplication functions of p53.
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below - 20°C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA is recommended for long-term storage.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet