Recombinant Human HspA9 (Hsp70 protein 9)

Recombinant Human HspA9 (Hsp70 protein 9)

ORDERING INFORMATION

Formulation: Sterile-filtered colorless solution in 20mM Tris-Hcl, pH 8.0, 0.5mM DTT and 10% glycerol. Purified by proprietary chromatographic techniques.

BACKGROUND

HspA9, a memer of the Hsp70 family, encodes a heat-shock cognate protein that is involved in the control of cell proliferation and acts as a chaperone. HspA9 is located on chromosome 5, band q31, a region thatis often deleted in myeloid leukemias and myelodysplasia, making it a candidate tumor suppressor gene. HspA9 suppresses nuclear translocation, transcriptional activation, and control centrosome-duplication functions of p53.

DESCRIPTION

Recombinant human HspA9 produced in E. coli is a single, non-glycosylated polypeptide containing 654 amino acids (aa 47-679) with a molecular weight of 71kDa. It is expressed with a 20-amino acid His-tag at the N-terminus.

SPECIFICATION SUMMARY

Source: Escherichia coli
Purity: Greater than 95% as determined by SDS-PAGE.
Accession number: P38646
Amino acid sequence:
GSSHHHHHH SSGLVPRGSH MASEAIKGAV VGIDLGTTNS CVAVMEGKQA KVLENAEGAR TTPSVVAFTA DGERLVGMPA KRQAVTNPNN TFYATKRLIG RRYDDPEVQK DIKNVPFKIV RASNGDAWVE AHGKLYSPSQ IGAFVLMKMK ETAENYLGHT AKNAVITVPA YFNDSQRQAT KDAGQISGLN VLRVINEPTA AALAYGLDKS EDKVIAVYDL GGGTFDISIL EIQKGVFEVK STNGDTFLGG EDFDQALLRH IVKEFKRETG VDLTKDNMAL QRVREAAEKA KCELSSSVQT DINLPYLTMD SSGPKHLNMK LTRAQFEGIV TDLIRRTIAP CQKAMQDAEV SKSDIGEVIL VGGMTRMPKV QQTVQDLFGR APSKAVNPDE AVAIGAAIQG GVLAGDVTDV LLLDVTPLSL GIETLGGVFT KLINRNTTIP TKKSQVFSTA ADGQTQVEIK VCQGEREMAG DNKLLGQFTL IGIPPAPRGV PQIEVTFDID ANGIVHVSAK DKGTGREQQI VIQSSGGLSK DDIENMVKNA EKYAEEDRRK KERVEAVNMA EGIIHDTETK MEEFKDQLPA DECNKLKEEI SKMRELLARK DSETGENIRQ AASSLQQASL KLFEMAYKKM ASEREGSGSS GTGEQKEDQK EEKQ.

STORAGE AND STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below – 20oC for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA is recommended for long-term storage.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.