Recombinant Human Hsp90 beta1

Recombinant Human Hsp90 beta1

ORDERING INFORMATION

Catalog Number	Size
11106P-2	2ug
11106P-10	10ug
11106P-1000	1000ug

 Format: Produced in E. coli as a single, non-glycosylated polypeptide containing 819 amino acids (aa 22-803) with a molecular weight of 94.4kDa. It is expressed with a 37 amino acid His-tag at the N-terminus. 

Sterile-filtered colorless solution in 20mM Tris-Hcl, pH 8.0, 1mM EDTA, 0.1M NaCl, 1mM DTT, and 10% glycerol. Purified by proprietary chromatographic techniques. 

BACKGROUND 

Hsp90β1, a member of the Hsp90 family of molecular chaperones, is involved in maintaining protein homeostasis in the secretory pathway and in intracellular trafficking of peptides from the extracellular space to the MHC class I antigen-processing pathway of antigen-presenting cells. Hsp90β1 has key roles in signal transduction, protein folding, and protein degradation. It associates with several co-chaperones in folding of newly synthesized proteins and refolding of denatured proteins after stress. Hsp90β1 is highly expressed in human gastric carcinoma BGC-823 cells throughout the cell cycle. 

SPECIFICATION SUMMARY 

Source: Escherichia coli 

Purity: Greater than 90% as determined by SDS-PAGE and RP-HPLC. 

Accession number: P14625 

Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMDDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV GTDEEEETAK ESTAEKDEL. 

STORAGE AND STABILITY 

Store at 4oC if entire vial will be used within 2-4 weeks. Store at or below -20oC for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage. 

For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.

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