Recombinant Human Hsp90 beta1

Recombinant Human Hsp90 beta1

ORDERING INFORMATION

Formulation: Sterile-filtered colorless solution in 20mM Tris-Hcl, pH 8.0, 1mM EDTA, 0.1M NaCl, 1mM DTT, and 10% glycerol. Purified by proprietary chromatographic techniques.

BACKGROUND

Hsp90β1, a member of the Hsp90 family of molecular chaperones, is involved in maintaining protein homeostasis in the secretory pathway and in intracellular trafficking of peptides from the extracellular space to the MHC class I antigen-processing pathway of antigen- presenting cells. Hsp90β1 has key roles in signal transduction, protein folding, and protein degradation. It associates with several co-chaperones in folding of newly synthesized proteins and refolding of denatured proteins after stress. Hsp90β1 is highly expressed in human gastric carcinoma BGC-823 cells throughout the cell cycle.

DESCRIPTION

Recombinant human Hsp90 produced in E. coli is a single, non-glycosylated polypeptide containing 819 amino acids (aa 22-8°C03) with a molecular weight of 94.4kDa. It is expressed with a 37 amino acid His-tag at the N-terminus.

SPECIFICATION SUMMARY

Source: Escherichia coli
Purity: Greater than 90% as determined by SDS-PAGE and RP- HPLC.
Accession number: P14625

STORAGE AND STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below -20°C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA is recommended for long-term storage.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.