Recombinant Human GroEL (Hsp60)

Recombinant Human GroEL (Hsp60)

ORDERING INFORMATION

Formulation: Sterile-filtered colorless solution contains 25mM Tris-HCl, pH 7.5, 100mM NaCl, 5mM DTT, and 10% glycerol. Purified by proprietary chromatographic techniques.

BACKGROUND

Hsp60 is a mitochondrial chaperonin responsible for transportation and refolding of proteins from the cytoplasm directly into the mitochondrial matrix. Hsp60 is regulated by the Hsp10 co- chaperonin. Hsp10 coordinates the ATPase activity of the Hsp60 subunits in order to allow the release of bound polypeptide in a manner that is productive for its correct folding.

DESCRIPTION

Recombinant Human GroEL (Hsp60) produced in E. coli is a single, non- glycosylated polypeptide chain containing 553 amino acids with a molecular weight of 63kDa.

SPECIFICATION SUMMARY

Source: Escherichia coli
Purity: Greater than 95% as determined by SDS-PAGE and RP- HPLC.
Accession number: P10809
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALMLQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQDVANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSKPVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF

STORAGE AND STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below -20°C for longer periods of time. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.