Recombinant Human GroEL (Hsp60)

$173.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
	5ug	WB	Human

SKU: 11100P-5 Categories: Products, Recombinant Proteins

Overview

Product Name GroEL (11100P-5)

Description Recombinant Human GroELHsp60) produced in E. coli is a single, non- glycosylated polypeptide chain containing 553 amino acids with a molecular weight of 63kDa.

Target GroEL

Product Background Hsp60 is a mitochondrial chaperonin responsible for transportation and refolding of proteins from the cytoplasm directly into the mitochondrial matrix. Hsp60 is regulated by the Hsp10 co- chaperonin. Hsp10 coordinates the ATPase activity of the Hsp60 subunits in order to allow the release of bound polypeptide in a manner that is productive for its correct folding.

Species Reactivity Human

Applications WB

Source Escherichia coli

Properties

Form Liquid

Concentration Lot Specific

Formulation Sterile-filtered colorless solution contains 25mM Tris-HCl, pH 7.5, 100mM NaCl, 5mM DTT, and 10% glycerol.

Buffer Formulation 25mM Tris-HCl, 100mM NaCl, 5mM DTT

Buffer pH pH 7.5

Buffer Cryopreservative 10% glycerol

Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC

Target Name Heat shock protein 60

Target ID GroEL

Alternative Names Hsp60

Sequence Location Mitochondrion matrix.

Sequence MGSSHHHHHH SSGLVPRGSH MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALMLQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQDVANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSKPVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF

Biological Function Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). {PubMed:11422376, PubMed:1346131, PubMed:25918392}.

Background Hsp60 is a mitochondrial chaperonin responsible for transportation and refolding of proteins from the cytoplasm directly into the mitochondrial matrix. Hsp60 is regulated by the Hsp10 co- chaperonin. Hsp10 coordinates the ATPase activity of the Hsp60 subunits in order to allow the release of bound polypeptide in a manner that is productive for its correct folding.

Handling

Storage Store at 2 - 8ºC. Do not Freeze.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Data Sheet