Recombinant Human Fibroblast Growth Factor – basic (rHuFGF-b)

Recombinant Human Fibroblast Growth Factor- basic

ORDERING INFORMATION

Format: Sterile filtered, lyophilized.
Purification: Chromatographic

DESCRIPTION

Recombinant Human FGF-basic produced in E. coli is a single, non- glycosylated polypeptide chain containing 155 amino acids with a molecular mass of 17,353 daltons. This product has full biological activity.

SPECIFICATION SUMMARY

Source: Escherichia coli
Stabilizers: None
Preservatives: None
Purity: Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Dimers/Aggregates: <1% by SDS- PAGE
Biological Activity: Fully biologically active compared to reference standards. Specific Activity is 2 x 106 Units/mg.
Endotoxin: <0.1 ng/ug
Protein Content: Determined spectrophotometrically at 280nm (0.8511 ext. coeff), and by RP-HPLC analysis against a calibrated standard.

SOLUBILITY

Reconstitute the lyophilized product in sterile water not less than 100 ug/ml. Product was dialyzed against 5mM Tris pH 7.5/150mM NaCl prior to lyophilization.

STORAGE AND STABILITY

Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze- thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.