Recombinant Human Cu/Zn Superoxide Dismutase (SOD1) Homodimer
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Formulation: Lyophilized from a sterile-filtered solution (1mg/ml) in PBS, pH 7.4 Purified by proprietary chromatographic techniques.
Cu/Zn Superoxide Dismutase (SOD1) catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. It protects a cell from dangerous levels of superoxide. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS).
Recombinant Human Cu/Zn SOD1 produced in E. coli is a homodimer non-glycosylated polypeptide chain containing 2 x 154 amino acids with a molecular weight of 31.6kDa.
Source: Escherichia coli
Purity: Greater than 95% as determined by SDS- PAGE and RP-HPLC.
Accession number: P00441.2
Amino acid sequence:
MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVIGIAQ
Reconstitute lyophilized product in sterile distilled H2O to no less than 100ug/ml which can be further diluted in other aqueous solutions as needed.
Potency per mg is 10,000 units/mg as determined by the Pyrogallic Acid method.
STORAGE AND STABILITY
Although lyophilized product is stable at room temperature for 3 weeks, it is best stored at or below -20°C. After reconstitution, product should be stored at or below -20°C. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.