Recombinant CCL27 (CTACK)
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CCL27, also known as Cutaneous T-cell-attracting Chemokine (CTACK) is a ligand for cell surface receptor CCR10. It is responsible for chemotaxis of skin-homing memory T-cells during cutaneous inflammation. Interestingly, after CCR10-mediated internalization, CCL27, as well as a non-secreted splice variant of the same gene, can reach the nucleus and modulate transcription and cell behavior.
Source: Recombinant human CCL27, produced in E. coli, corresponds to aa 25- 112 (accession no. Q9Y4X3).
Protein Sequence: FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQ RSICIHPQNPSLSQWFEHQERKLHGTLPKLNFGMLRKMG
Molecular Mass: 10.149kDa by Mass Spec.
Activity: EC50 = 34nM determined by Migration Assay in L1.2 cells expressing CCR10.
Endotoxin Level: <0.01 EU per 1ug of protein by LAL method. Form: Lyophilized. Carrier Protein: None.
PREPARATION AND STORAGE
Reconstitution: Recommended at 100ug/ml in sterile distilled water.
Stability and Storage: 12 months from date of receipt, -20°C to -70°C, as supplied.
1 month, 2oC to 8oC, under sterile conditions after reconstitution.
3 months, -20°C to -70°C, under sterile conditions after reconstitution.
For in vitro investigational use only. Not for use in diagnostic or therapeutic procedures.