Recombinant Human Annexin A7

Recombinant Human Annexin A7 (ANXA7)

ORDERING INFORMATION

Formulation: Sterile-filtered clear solution (0.25mg/ml) in 20mM Tris-HCl, pH 8.0, 150mM NaCl,1mM DTT, 40% glycerol. Purified by proprietary chromatographic techniques.

BACKGROUND

Annexin 7 is a member of the annexin family of calcium-dependent phospholipids binding proteins. It includes a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter is composed of alternating hydrophobic and hydrophilic segments. Analysis of this protein suggests that Annexin 7 is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity, and membrane fusion.

DESCRIPTION

Recombinant Human Annexin A7 produced in E. coli is a single, non- glycosylated polypeptide containing amino acids 1-466 with a molecular weight of 52.9kDa. It includes a 24 amino acid His-tag at the N-terminus.

SPECIFICATION SUMMARY

Source: Escherichia coli Purity: Greater than 85% as determined by SDS-PAGE. Accession number: P20073.3 Amino acid sequence: MGSSHHHHHH SSGLVPRGSH

STORAGE AND STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. Store at -20°C for longer periods of time. For long-term storage, addition of 0.1% human serum albumin or bovine serum albumin is recommended. Avoid multiple freeze- thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.