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CCL5, biotinylated (50181PB-50)

Host Quantity Applications Species Reactivity Data Sheet  
50ug Migration assay Human
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SKU: 50181PB-50 Categories: ,
Overview
Product Name CCL5, biotinylated (50181PB-50)
Description Recombinant human CCL5, produced in E. coli, corresponds to aa 24-91
Target CCL5ylated
Species Reactivity Human
Applications Migration assay
Conjugate Biotin
Associated Products #1112 Anti-HIV Co-Receptor CCR5 Antibody 50181PB-2 2ug
Source Recombinant human CCL5, produced in E. coli
Properties
Form Lyophilized
Purity >97% by SDS-PAGE
Background CCL5, also known as Regulated on Activation, Normal T-cell Expressed and Secreted (RANTES), is a proinflammatory chemokine that induces migration and activation of leukocytes, and is also implied in HIV infection. CCL5 binds to cell surface receptors CCR1, CCR3, CCR4, and CCR5. Its biological effects on leukocyte activation and HIV infections is dependent upon concentration and on the binding of cell surface glycosaminoglycans.
Specificity Information
Target Name C-C motif chemokine 5
Target ID CCL5ylated
Alternative Names rHuCCL5-biotin, Rantes
Sequence Location Secreted.
Sequence SPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQV CANPEKKWVREYINSLEMS
Biological Activity CCL5ylated
Biological Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. RPubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:17001303, PubMed:23979485, PubMed:8525373, PubMed:9516414}.
Background CCL5, also known as Regulated on Activation, Normal T-cell Expressed and Secreted (RANTES), is a proinflammatory chemokine that induces migration and activation of leukocytes, and is also implied in HIV infection. CCL5 binds to cell surface receptors CCR1, CCR3, CCR4, and CCR5. Its biological effects on leukocyte activation and HIV infections is dependent upon concentration and on the binding of cell surface glycosaminoglycans.
Application Images
Additional Information
Additional Information Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration Assay in cells expressing recombinant CCR5.
Handling
Storage 12 months from date of receipt, -20°C to -70°C, as supplied. 1 month, -20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution.
Dilution Instructions Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.
References & Data Sheet