Recombinant CCL4 (MIP-1β)
Catalog No., Size:
CCL4, also known as Macrophage Inflammatory Protein-1beta (MIP-1β) is a small cytokine (~9kDa) that belongs to the CC chemokine family. CCL4 binds to CCR1, CCR2 isoform B, and CCR5. CCL4 is one of the major HIV-suppressive factors produced by CD8+ T-cells; recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form of MIP-1β (aa 3-69) retains the abilities to induce down-modulation of surface expression of CCR5 and to inhibit CCR5-mediated entry of HIV-1 into T-cells.
Source: Recombinant human CCL4 is produced in E. coli (accession no. P13236, aa 24-92).
Protein Sequence: APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTK RSKQVCADPSESWVQEYVYDLELN
Molecular Mass: 7.818kDa by Mass Spec.
Purity: >97% by SDS-PAGE
Activity: EC50 = 0.45-0.63nM determined by Migration Assay in cells expressing recombinant CCR5.
Endotoxin Level: <0.01 EU per 1ug of protein by LAL method. Form: Lyophilized. Carrier Protein: None.
PREPARATION AND STORAGE
Reconstitution: Recommended at 100ug/ml in sterile distilled water.
Stability and Storage: 12 months from date of receipt, -20°C to -70°C, as supplied.
1 month, -20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution.
Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in diagnostic or therapeutic procedures.