Recombinant CCL2 (MCP-1)

$2,454.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
	1000ug	Migration assay	Human

SKU: 50186P-1000 Categories: Chemokines, Products

Overview

Product Name CCL2 (50186P-1000)

Description Recombinant human CCL2 is produced in E. coli

Target CCL2

Product Background CCL2, also known as Monocyte Chemoattractant Protein-1 (MCP-1) is a small cytokine (~13kDa) that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. To become activated, CCL2 protein has to be cleaved by metalloproteinase MMP-12. CCL2 is implicated in the pathogenesis of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCR2 and CCR4 are two cell surface receptors that bind CCL2.

Species Reactivity Human

Applications Migration assay

Additional Information Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration or recombinant CCR2- expressing cells.

Source Recombinant human CCL2 is produced in E. coli

Properties

Form Lyophilized.

Concentration Lot Specific

Molecular Mass Molecular Mass: 8.662 kDa

Purity >97% by SDS-PAGE

Target Name C-C motif chemokine 2

Target ID CCL2

Alternative Names rHuCCL2, MCP-1

Sequence Location Secreted

Sequence QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAK EICADPKQKWVQDSIDHLDKQTQTPKTMolecular Mass8.662kDa by Mass Spec.Purity>97% by SDS-PAGEActivityEC50 = 0.067nM determined by migration or recombinant CCR2- expressing cells.Endotoxin Level<0.01 EU per 1ug of protein by LAL method. FormLyophilized. Carrier ProteinNone.

Biological Activity CCL2

Biological Function Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690). {PubMed:10529171, PubMed:10587439, PubMed:8107690, PubMed:8195247, PubMed:8627182, PubMed:9792674, PubMed:9837883}.

Background CCL2, also known as Monocyte Chemoattractant Protein-1 (MCP-1) is a small cytokine (~13kDa) that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. To become activated, CCL2 protein has to be cleaved by metalloproteinase MMP-12. CCL2 is implicated in the pathogenesis of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCR2 and CCR4 are two cell surface receptors that bind CCL2.

Handling

Storage Store at 2 - 8ºC. Do not Freeze.

Dilution Instructions Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.

Data Sheet