Recombinant CCL19 (MIP-3β), biotinylated
Catalog No., Size:
CCL19 (also known as Macrophage Inflammatory Protein-3beta, MIP-3β, and EBI1 ligand chemokine, ELC) directs chemotaxis of dendritic cells and certain B- and T-lymphocytes, but not monocytes or granulocytes. It is constitutively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. As a homeostatic chemokine, its primary physiological role is thought to be in the normal recirculation and homing of lymphocytes. However, CCL19 can also be proinflammatory and is implicated in post-HIV infection responses.
Source: Recombinant human CCL19 produced in E. coli (accession no. Q99731, aa 22-98).
Protein Sequence: GTNDAEDCCLSVTQKPIPGYIVRNFHYLLIKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS
Molecular Mass: 11.22kDa by Mass Spec.
Purity: >97% by SDS-PAGE
Activity: EC50 = 18-25nM determined by Migration Assay with cells expressing recombinant CCR7.
Endotoxin Level: <0.01 EU per 1ug of protein by LAL method. Form: Lyophilized. Carrier Protein: None.
PREPARATION AND STORAGE
Reconstitution: Recommended at 100ug/ml in sterile distilled water.
Stability and Storage: 12 months from date of receipt, -20°C to -70°C, as supplied.
1 month,- 20oC to -70°C, under sterile conditions after reconstitution.
Best if used immediately after reconstitution.
Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in diagnostic or therapeutic procedures.