RbAp48 Monoclonal Antibody
Catalog no.: 3111
Format: 100ug Protein G-purified antibody in PBS, pH 7.4.
RbAp48, product of the RBBP4 gene in humans, is a 48 kDa nuclear protein that was first identified through its interaction with the carboxyl-terminus of retinoblastoma protein (Rb). In mice, the RbAp48 protein is a key component in histone acetylation, transcriptional regulation and in the cyclic adenosine monophosphate (cAMP)-protein kinase element-binding protein CREB1 path way. Since histone acetylation and the cAMP-PKA-CREB1 pathway are extremely important for normal hippocampal function, RbAp48 has been investigated to test whether decreased expression of RbAp48 in aged animals is directly associated with age-related memory loss.
Antigen: The complete coding region (aa 1-425) of RbAp48 expressed in E. coli.
Accession no.: Q09028
Gene ID: 5928
Antibody Class: IgG2a
Clone no.: 15G12
Host Species: Mouse
Specificity: This antibody recognizes human and mouse RbAp48 and RbAp46.
Immunoblotting: use at 1-5ug/ml. A band of ~48kDa is detected.
Detection of RbAp48 in K562, THP-1, and HL-60 whole cell extracts (30ug), separated by 10% SDS-PAGE, with #3111 at 2ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Immunohistochemistry: use at 5-10ug/ml.
Detection of RbAp48 in paraffin-embedded human cervical carcinoma with #3111 at 10ug/ml.
STORAGE AND STABILITY
This antibody is stable at -20ºC for at least one (1) year; store in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.