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Anti-Rat IgG2a Antibody, Alkaline Phosphatase conjugate

$267.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 0.5mg WB,IHC,ICC,ELISA Rat
SKU: 88402 Categories: , ,
Overview
Product Name Anti-Rat IgG2a Antibody, Alkaline Phosphatase conjugate (88402)
Description Anti-Rat IgG2a-Alk Phos Goat Polyclonal Antibody
Target Rat IgG2a, Alkaline Phosphatase conjugate
Species Reactivity Rat
Applications WB,IHC,ICC,ELISA
Host Goat
Clonality Polyclonal
Isotype Whole IgG
Conjugate Alkaline phosphatase
Immunogen Rat IgG2a
Properties
Form Liquid
Concentration 0.5 mg/mL
Formulation 50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3
Buffer Formulation HEPES
Buffer pH pH 7.1
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Antibody is affinity-purified on rat IgG2a and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG2a of other species.
Target ID Rat IgG2a, Alkaline Phosphatase conjugate
Research Areas Secondary Antibodies
Application Images
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:10,000.

Immunohistochemistry: use at a dilution of 1:100-1:1,000

Immunocytochemistry: use at a dilution of 1:100-1:1,000

ELISA: use at a dilution of 1:1,000-1:10,000.

These are recommended dilutions.

End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.
References & Data Sheet