Raf1 (Phospho-Ser259) Polyclonal Antibody
Catalog No.: 43006
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
RAF proto-oncogene serine/threonine-protein kinase, also known as Raf1, is part of the ERK1/2 pathway as a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. Raf1 is a member of the Raf kinase family of serine/threonine-specific protein kinases from the TKL (Tyrosine-kinase-like) group of kinases.
Antigen: Peptide sequence that includes phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from human Raf1 and conjugated to KLH.
Accession no.: P04049, NP_002871.1
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat Raf1 only when phosphorylated at serine 259.
Western blotting: use at dilution of 1:500- 1:1,000. A band of ~73kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.