Rad51 Monoclonal Antibody
Catalog No.: 56214
Size: 100ul purified antibody in PBS, pH 7.4
Antigen: GST fusion protein corresponding to full-length Rad51 (aa 1-338) of human Rad51 expressed in E. coli.
Host Species: Mouse
Antibody Class: IgG2b/κ
Preservatives: None. Available on request.
This antibody recognizes human Rad51, a 37 kD protein that interacts with BRCA1. This interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact via the BRC repeats located with exon 11 of BRCA2. When these repeats are deleted, the interaction is lost, and cells become hypersensitive to DNA damage caused by methyl methanesulfonate (MMS).
Immunoblotting, immunoprecipitation: use at 1-5 ug/ml.
Positive control: T24 bladder carcinoma cells and recombinant fusion protein.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Shinohara, A et al. 1993. Nature Genetics 4: 239-243;
Scully, R et al. 1997. Cell 88: 265-275;
Chen, PL et al. 1998. PNAS (USA) 95: 5287-5292.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.