Product Name Anti-RAD17 Antibody (56229)
Description Anti-RAD17 Rabbit Polyclonal Antibody
Species Reactivity Human
Immunogen Peptide corresponding to aa 2-15 of human RAD17.
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Purification Purified by peptide immuno-affinity chromatography
Specificity This antibody recognizes human RAD17, a novel gene product involved in the DNA damage checkpoint signal transduction pathway. RAD17 plays a central role in cell cycle checkpoint control.
Target Name Cell cycle checkpoint protein RAD17
Target ID RAD17
Uniprot ID O75943
Alternative Names hRad17, RF-C/activator 1 homolog
Gene Name RAD17
Sequence Location Nucleus
Biological Function Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous rPubMed:10208430, PubMed:11418864, PubMed:11687627, PubMed:11799063, PubMed:12578958, PubMed:12672690, PubMed:14500819, PubMed:14624239, PubMed:15235112, PubMed:15538388, PubMed:21659603}.
Research Areas Cancer research
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution. A band of approx. 69 kD is detected.