Product Name Anti-PSE1/Ku Protein Antibody (70104)
Description Anti-PSE1/Ku Protein Mouse Monoclonal Antibody
Target PSE1/Ku Protein
Species Reactivity Human
Applications WB,Gel Shift
Clone ID N3H10
Immunogen Proximal sequence element (PSE1) of the human U1 small nuclear RNA extracted from human placenta.
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Purification Purified by Protein G affinity chromatography
Specificity 70104 recognizes the small b subunit (~70 kDa) and 70105 recognizes the large alpha subunit (~80-90 kDa) of placental PSE1 and human Ku in solution and when bound to DNA.
Target Name X-ray repair cross-complementing protein 6
Target ID PSE1/Ku Protein
Uniprot ID P23475
Alternative Names EC 3.6.4.-, EC 4.2.99.-, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP/AP lyase Ku70, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, Ku autoantigen protein p70 homolog, Ku70
Gene Name Xrcc6
Sequence Location Nucleus, Chromosome
Biological Function Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (By similarity). Required for double-strand break repair and V(D)J rUniProtKB:P12956, PubMed:12145306}.
Research Areas Protein Transcription
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies work well in Western blot and electrophoretic mobility (gel shift) assays for identifying PSE1 or Ku. They may be used to immuno-deplete Ku protein from protein mixtures prior to specific DNA affinity chromatography.