Protocadherin Gamma A (pan) Monoclonal Antibody
Catalog no.: 56560 (clone S144-32)
Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. The gamma gene cluster includes 22 genes divided into 3 subfamilies: subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region (which includes 6 cadherin ectodomains and a trans-membrane region). The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins are believed to play a critical role in the establishment and function of specific cell-cell connections in the brain.
Antigen: Fusion protein corresponding to aa 720-804 (variable cytoplasmic domain) of mouse PCDHGA3. This sequence is 80% identical to human and >75% identical to other Protocadherin Gamma A proteins.
Accession nos.: NP_291064.1, Q91XY5
Gene ID: 93711
Host Species: Mouse
Antibody Class: IgG2b
Specificity: This antibody recognizes human, mouse, and rat PCDHGA proteins. It does not cross-react with Protocadherin Gamma B or C proteins.
Immunoblotting: use at 1-5ug/ml. A band of ~100kDa is detected.
Immunofluoresence: use at 10ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their application.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.