Prostaglandin D2 ELISA Kit (ECP7922)
$711.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Prostaglandin D2 ELISA Kit (ECP7922)
Description Prostaglandin D2 ELISA Kit (ECP7922) is a Competitive Inhibition ELISA for the quantitiation of Prostaglandin D2 in a variety of aqueous sample types.
Target Prostaglandin D2
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 352.465 g/mol
Background Minimum Level of Detection: 4.61 pg/mL
Dynamic range of quantitation: 12.35-1,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 12.35-1,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Prostaglandin D2 Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Prostaglandin D2 Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 Vial |
| Resuspension Solution A | 1 x 300uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| Stop Solution | 1 x 6mL |
| TMB Substrate | 1 x 9mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Prostaglandin D2
Alternative Names 9alpha,15S-dihydroxy-11-oxo-prosta-5Z,13E-dien-1-oic acid
CAS Number 41598-07-6
PubChem ID 448457
Research Areas Biochemicals
Background Prostaglandin D2 is a member of the class of prostaglandins D that is prosta-5,13-dien-1-oic acid substituted by hydroxy groups at positions 9 and 15 and an oxo group at position 11 (the 5Z,9alpha,13E,15S- stereoisomer). It has a role as a human metabolite and a mouse metabolite. It is a conjugate acid of a prostaglandin D2(1-).
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.