Progesterone Receptor (Phospho-Ser190) Polyclonal Antibody
Catalog No.: 43074
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
Progesterone receptor (PR) is a nuclear hormone receptor of the NR3C class which includes mineralocorticoid, glucocorticoid, and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins which are shed upon activation. Progesterone receptors signal directly via DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins with transcription factors such as NF-kappa B, AP-1, or STAT. Progesterone receptor expression is induced by estrogen, and the human gene encoding the progesterone receptor has been localized to 11q22.
Antigen: Peptide sequence that includes phosphorylation site of serine 190 (G-L-S(p)-P-A) derived from human progesterone receptor and conjugated to KLH.
Host Species: Rabbit
Specificity: This antibody detects endogenous human progesterone receptor only when phosphorylated at serine 190.
Accession no.: P06401, NP_000917.3
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected.
Immunofluorescence: use at dilution of 1:100-1:200.
Immunohistochemistry: use at dilution of 1:50-1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.