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Anti-PKD/PKCμ (phospho-Ser738) Antibody (43078)

$355.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IF Human, Mouse, Rat
SKU: 43078 Categories: , ,
Overview
Product Name Anti-PKD/PKCμ (phospho-Ser738) Antibody (43078)
Description Anti-PKD/PKCμ (phospho-Ser738) Rabbit Polyclonal Antibody
Target PKD/PKCμ (phospho-Ser738)
Species Reactivity Human, Mouse, Rat
Applications WB,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 738 (E-K-S(p)-F-R) derived from human PKD/PCKu and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat PKD/PKCu only when phosphorylated at serine 738.Accession no.: Q15139, NP_002733.2
Target ID PKD/PKCμ (phospho-Ser738)
Post Translational Modification PKD/PKCμ (phospho-Ser738)
Target Phospho-ser738
Research Areas Phosphospecific Antibodies
Background Protein kinase C (PKC), a family of lipid-activated serine kinases, is involved in multiple functions in the regulation of growth control. The PKC-related isoform PKC mu/PKD has been implicated in mitogenic signal cascades because of the activation of p42/p44 MAPK leading to Elk1-mediated gene transcription, and PKC mu/PKD has been shown to be activated via a PKC-dependent pathway.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500- 1:1,000. A band of ~115kDa is detected.

Immunofluorescence: use at dilution of 1:100- 1:200.

These are recommended working dilutions.

End users should determine optimal dilutions for their applications.
References & Data Sheet