Anti-PID/MTA2 Antibody (56227)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF,IHC-P Human, Mouse, Rat
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Overview
Product Name Anti-PID/MTA2 Antibody (56227)
Description Anti-PID/MTA2 Rabbit Polyclonal Antibody
Target PID/MTA2
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF,IHC-P
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to aa 652-668 of human PID/MTA2. This sequence differs from that of mouse by one amino acid.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat PID/MTA2, a subunit of the nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex, and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53- dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is expressed in a variety of tissues. This antibody does not cross-react with MTA1.
Target Name Metastasis-associated protein MTA2
Target ID PID/MTA2
Uniprot ID O94776
Alternative Names Metastasis-associated 1-like 1, MTA1-L1 protein, p53 target protein in deacetylase complex
Gene Name MTA2
Sequence Location Nucleus
Biological Function May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
Research Areas Cancer research
Application Images
Description Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 ug/mL.
Description Immunocytochemistry staining of HeLa using PID antibody at 10 ug/mL.
Description Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.
Description Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 ug/ml.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 75 kD is detected.

Positive control: HeLa cell lysate.
References & Data Sheet