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Anti-Piccolo Antibody (56496)

$422.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human, Mouse, Rat
SKU: 56496 Categories: , ,
Overview
Product Name Anti-Piccolo Antibody (56496)
Description Anti-Piccolo Rabbit Polyclonal Antibody
Target Piccolo
Species Reactivity Human, Mouse, Rat
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Full-length human protein (accession no. BC001304.1)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Piccolo isoforms (other species not tested). It does not cross-react with Bassoon.
Target ID Piccolo
Uniprot ID Q9Y6VO
Research Areas Neuroscience
Background Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin- mediated endocytosis by binding to the F- actin and dynamin binding protein Abp1.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 10ug/ml. A band of ~550kDa is detected

ELISA: use at 1-10ug/ml with Piccolo on the solid phase.

Positive control: Rat brain tissue lysate

These are recommended concentrations

End user should determine optimal concentrations for their applications.
References & Data Sheet