Anti-PfHsp60 Antibody (11146)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF,IHC P. Facilparum
SKU: 11146 Categories: , , ,
Overview
Product Name Anti-PfHsp60 Antibody (11146)
Description Anti-PfHsp60 Rabbit Polyclonal Antibody
Target PfHsp60
Species Reactivity P. Facilparum
Applications WB,ICC/IF,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Recombinant full-length PfHsp60 (Accession no. XM_001347402.1)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS (1.83mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes P. falciparum Hsp60 and cross-reacts with E.coli Hsp60/GroEl.
Target Name Chaperonin CPN60, mitochondrial
Target ID PfHsp60
Uniprot ID P34940
Accession Number XM_001347402.1
Sequence Location Mitochondrion matrix
Biological Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Research Areas Heat Shock& Stress Proteins
Background Hsp60 is an abundant, constitutively produced heat shock protein that increases in cellular concentration in response to cell shock. It is found in multiple species and is notably homologous in bacteria, plants, and mammals. Hsp60 of Plasmodium falciparum is a polypeptide of 577 amino acids with a calculated molecular weight of 62158 Da. The primary structure of P. falciparum hsp60 contains a putative mitochondrial targeting peptide at its amino- terminus and a GGM motif at its carboxyl- terminus. The overall structure exhibits strong conservation (approximately 50%) to the hsp60 from human and other eukaryotes, but only low homology (< 30%) to a recently reported P. falciparum chaperonin 60 gene. The P. falciparum hsp60 gene is located on chromosome 10. During heat shock, the level of hsp60 transcript in blood stage parasites increases significantly and its accumulation correlates with the duration of the induction.
Application Images
Description Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody (11146). Tissue: Liver. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (11146).
Description Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (11146). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (11146) at 1:1000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~62kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet