PDK1 (Phospho-Ser241)

PDK1 (Phospho-Ser241) Polyclonal Antibody

ORDERING INFORMATION

Catalog No.: 43005
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.

BACKGROUND

PDK1 phosphorylates and activates PKB/AKT, PKA, PKC-zeta, RPS6KA1, and RPS6KB1 and plays a role in general signaling processes and in development.

SPECIFICATION SUMMARY

Antigen: Peptide sequence that includes phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from human PDK1 and conjugated to KLH.
Accession no.: O15530, NP_002604.1
Host Species: Rabbit
Specificity: This antibody detects human, mouse, and rat endogenous PDK1 only when phosphorylated at serine 241.

APPLICATIONS

Western blotting: use at dilution of 1:500- 1:1,000. A band of ~63kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.

For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.

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