Mikrogen Diagnostik recomWell Parvovirus B19 IgM ELISA Kit (4405)

Host Quantity Applications Species Reactivity Data Sheet  
96 Tests ELISA Parvovirus B19
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Overview
Product Name Mikrogen Diagnostik recomWell Parvovirus B19 IgM ELISA Kit (4405)
Description Enzyme immunoassay with antigens produced by recombinant techniques for the detection of IgG and IgM antibodies against the human Parvovirus B19

Product is intended for research use.
Target Human Parvovirus B19 IgM
Species Reactivity Parvovirus B19
Assay Type Indirect ELISA
Applications ELISA
Properties
Background Human Parvovirus B19 occurs endemically worldwide. At intervals of several years, it causes epidemics in small areas from late fall to early summer, affecting kindergartens and schools in particular. The infection rate in children is between 15 and 30 % and in adults about 60 %.The virus has a single stranded genome of about 5.6 kB and is packed in an icosahedral capsid, without a lipid layer, with a diameter of 22 nm. The two capsid proteins VP-1 and VP-2 are coded from the same reading frame. VP-1 is distinguished from VP-2 only by an additional N-terminal fragment. Furthermore, a nonstructural protein NS-1 is synthesized, which is required for virus replication and propagation in the cell.Clinical spectrum of a Parvovirus B19 infection:Fifth disease (erythema infectiosum): It progresses in 20 % of cases without symptoms or with flu-like symptoms. Apart from the exanthema, polyarthralgia and a generalized swelling of the lymph nodes can occur occasionally.Aplastic crisis in patients suffering from hemolytic anemias.Spontaneous abortion/hydrops fetalis after infection during pregnancy.Persistent infection in immunosuppressed persons with chronic anemia and bone marrow depression.Genetically engineered VP-1 antigen as well as VP-2 particles are used in the recomWell Parvovirus B19 tests. With the use of VP-2 particles the presentation of conformational epitopes in addition to the linear epitopes is achieved.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
Dilution Buffer125 mL
TMB Substrate12 mL
Stop Slution12 mL
Instuctions for Use1 Each
Evaluation Form1 Each
Microplate Sealing Tape2 Each
Microtiter Plate12 x 8 Wells
Positive Control450 uL
Cut-Off Control450 uL
Negative Control450 uL
Anti-Human IgM Conjugate500 uL
Specificity Information
Target ID Human Parvovirus B19 IgM
Research Areas Infectious Disease
Application Images
Description Test Principle and Procedure:
Indirect sandwich test. Recombinant antigens are bound to the solid phase.

1. 1st Incubation. Add patient samples diluted 1:101 (sample: 10 µl of serum or plasma), incubate for 1 h at 37 °C.
2. Wash 4 times
3. 2nd Incubation
4. Add peroxidase conjugated anti-human IgG or IgM antibodies (conjugate), incubate for 30 min at 37 °C.
5. Wash 4 times
6. Color reaction. Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm
Description Signal strength of the recomWell Parvovirus B19 in comparison to two other, commercially available ELISAs. A series of dilutions of the Parvovirus B19 WHO standard was used as samples.
Description Blood donor sera.
Description Positive Sera
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet