Anti-PARK7 Antibody (28110)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ug | WB | Human, Mouse |  |
Overview
Product Name Anti-PARK7 Antibody (28110)
Description Anti-PARK7 (DJ-1) Rabbit Polyclonal Antibody
Target PARK7
Species Reactivity Human, Mouse
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 167-189 (AIVEALNGKEVAAQVKAPLVLKD) of human PARK7 conjugated to KLH at the N-terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse PARK7 (DJ-1). Sequence homology to mouse and rat DJ-1 is 82%.
Target Name Parkinson disease protein 7
Target ID PARK7
Uniprot ID Q99497
Alternative Names Maillard deglycase, Oncogene DJ1, Parkinsonism-associated deglycase, Protein DJ-1, DJ-1, Protein/nucleic acid deglycase DJ-1, EC 3.1.2.-, EC 3.5.1.-, EC 3.5.1.124
Gene Name PARK7
Sequence Location Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum
Biological Function Multifunctional protein with controversial molecular function which plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785, PubMed:26995087, PubMed:28993701). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:12612053, PubMed:15502874, PubMed:14749723, PubMed:17015834, PubMed:21097510, PubMed:18711745). Has been described as a protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed:25416785, PubMed:28596309). But this function is rebuted by other works (PubMed:27903648, PubMed:31653696). As a protein deglycase, repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed:25416785, PubMed:28013050, PubMed:26995087). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed:28596309). Protects histones from adduction by methylglyoxal, controls the levels of methylglyoxal-derived argininine modifications on chromatin (PubMed:30150385). Able to remove the glycations and restore histone 3, histone glycation disrupts both local and global chromatin architecture by altering histone-DNA interactions as well as histone acetylation and ubiquitination levels (PubMed:30150385, PubMed:30894531). Displays a very low glyoxalase activity that may reflect its deglycase activity (PubMed:22523093, PubMed:31653696, PubMed:28993701). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:19229105, PubMed:16632486). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting (By similarity). Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed:18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis (By similarity). {UniProtKB:Q99LX0, PubMed:11477070, PubMed:12612053, PubMed:12855764, PubMed:12939276, PubMed:14749723, PubMed:15181200, PubMed:15502874, PubMed:15976810, PubMed:16390825, PubMed:17015834, PubMed:18626009, PubMed:18711745, PubMed:19229105, PubMed:20186336, PubMed:20304780, PubMed:21097510, PubMed:22523093, PubMed:23792957, PubMed:23847046, PubMed:25416785, PubMed:26995087, PubMed:28013050, PubMed:28596309, PubMed:28993701, PubMed:30150385, PubMed:30894531, PubMed:9070310}.
Research Areas Neuroscience
Background PARK7 is one of four chromosomal loci (PARK2, PARK6, PARK7 and PARK9) known to be associated with autosomal recessive, early onset parkinsonism. Positional cloning within the refined PARK7 critical region recently identified mutations in the DJ-1 gene in PARK7- linked families. Mutations in the PARK7/DJ-1 gene cause autosomal- recessive Parkinsons disease.
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1:500 dilution. A band of approximately 19 kDa is detected.
Positive control: HEK293 cell lysate or mouse brain tissue lysate.
Positive control: HEK293 cell lysate or mouse brain tissue lysate.
References & Data Sheet
References Bandmann, O (2004) Neurology 62:357-3588.
Hedrich, K et al. (2004) Neurology 62: 389-94.
Bandopadhyay, R et al. (2004) Brain 127:420-30.
Bonifati V et al. (2003) Neurol Sci 24:159-60.
Hedrich, K et al. (2004) Neurology 62: 389-94.
Bandopadhyay, R et al. (2004) Brain 127:420-30.
Bonifati V et al. (2003) Neurol Sci 24:159-60.
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet