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Anti-P73 (Phospho-Tyr99) Antibody (43058)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB Human
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SKU: 43058 Categories: ,
Overview
Product Name Anti-P73 (Phospho-Tyr99) Antibody (43058)
Description Anti-P73 (Phospho-Tyr99) Rabbit Polyclonal Antibody
Target P73 (Phospho-Tyr99)
Species Reactivity Human
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 99 (S-P-Y(p)-A-Q) derived from human p73.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human p73 only when phosphorylated at tyrosine 99.
Target ID P73 (Phospho-Tyr99)
Post Translational Modification P73 (Phospho-Tyr99)
Target Phospho-tyr99
Research Areas Phosphospecific Antibodies
Background Tumor protein p73 is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another which allows p63 and p73 to transactivate p53- responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~80kDa is detected.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet