Anti-p38alpha MAP Kinase Monoclonal Antibody
Catalog No.: 56450 (clone 9F12)
Size: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
The MAPKs (Mitogen-Activated Protein Kinases) are protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. Upon activation, p38α MAPK translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. p38α MAPK is activated by chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, lipopolysaccharide, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs.
Antigen: Recombinant human p38α MAPK expressed in E. coli.
Host Species: Mouse
Antibody Class: IgG1
Preservatives: 0.09% sodium azide
Other additives: 50% glycerol
This antibody recognizes human p38α MAPK.
Immunoblotting: use at 1ug/ml. A band of ~38 kDa is detected.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Purified p38α MAPK.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.