Otoferlin Monoclonal Antibody
Catalog No.: 90023 (clone 13A9) Format: 100ug, Protein A-purified, lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Otoferlin protein is the product of the OTOF gene. Otoferlin is present in the brain and the cochlea of the inner ear where sound is processed. Hearing relies on Ca2+ influx-driven exocytosis of synaptic vesicles at the ribbon-type active zones of hair cells. The presence of several C2 domains in otoferlin, some of which bind Ca2+, suggest that otoferlin is a Ca2+ sensor for fusion in inner hair cell (IHC) synapses.
Antigen: GST fusion protein corresponding to aa 1-395 of human otoferlin (accession no. Q9HC10).
Host species: Mouse.
Antibody Class: IgG1.
This antibody recognizes human otoferlin.
Immunoblotting: use at 1-5ug/ml. A band of ~220kDa is detected.
Immunofluorescence: use at 2-10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Reconstitute in distilled water.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.