Anti-Osteonectin/SPARC Antibody (58008)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | WB,IHC,FACS | Human, Rabbit, Bovine, Porcine |  |
Overview
Product Name Anti-Osteonectin/SPARC Antibody (58008)
Description Anti-Osteonectin (SPARC) Mouse Monoclonal Antibody
Target Osteonectin/SPARC
Species Reactivity Human, Rabbit, Bovine, Porcine
Applications WB,IHC,FACS
Host Mouse
Clonality Monoclonal
Clone ID ON1-1
Isotype IgG1
Immunogen Bovine bone osteonectin (SPARC)
Properties
Form Lyophilized
Formulation 10 mM PBS, pH 7.4, 1% BSA, lyophilized
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody reacts with human, bovine, rabbit, and porcine bone-derived and platelet derived osteonectin as well as with thrombin-stimulated platelets.
Target Name SPARC
Target ID Osteonectin/SPARC
Uniprot ID P09486
Alternative Names Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine
Gene Name SPARC
Sequence Location Secreted, extracellular space, extracellular matrix, basement membrane
Biological Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Research Areas Cell adhesion
Handling
Storage This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored. Avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration).
Application Instructions ELISA: 1-10ug/ml with immobilized antigen
Western Blot: 1-10ug/ml, reducing and non-reducing conditions.
Immunohistochemistry: 1-10ug/ml, paraffin-embedded and frozen tissue sections.
Western Blot: 1-10ug/ml, reducing and non-reducing conditions.
Immunohistochemistry: 1-10ug/ml, paraffin-embedded and frozen tissue sections.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet