Bovine Osteocalcin Monoclonal Antibody
Catalog No.: 50114
Clone No.: OC4-30
Format: Antibody was purified by column chromatography, dissolved in 10mM PBS, pH7.4, containing 1.0% bovine serum albumin, and then lyophilized. The lyophilized antibody does not contain preservative.
Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation.
Antigen: Bovine osteocalcin
Host Species: Mouse
Antibody Subtype: IgG2a
Specificity: The γ-carboxylated residue at position 17; human, bovine, rat, rabbit, pig, dog, goat, sheep, and chicken.
Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120.
Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions.
Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval.
Endusers should determine optimal concentrations for their applications.
Dissolve lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration) then dilute further for applications in PBS or media that is identical to that used in the assay system.
STORAGE AND STABILITY
Store stock solution (2.0 mg/ml) in aliquots at -20℃ for 1 year, or at 4℃ for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.