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Anti-NPFF2 Antibody (56111)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human
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SKU: 56111 Categories: , ,
Overview
Product Name Anti-NPFF2 Antibody (56111)
Description Anti-NPFF2 Rabbit Polyclonal Antibody
Target NPFF2
Species Reactivity Human
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen N-terminal sequence MNEKWDTNSSENWHPI and C-terminal sequence ELVMEELKETTNSSEI of human NPFF2.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS.
Buffer Formulation Phosphate Buffered Saline
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody recognizes human NPFF2 (45 kD).
Target Name Neuropeptide FF receptor 2
Target ID NPFF2
Uniprot ID Q9Y5X5
Alternative Names G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, Neuropeptide G-protein coupled receptor
Gene Name NPFFR2
Sequence Location Cell membrane; Multi-pass membrane protein.
Biological Function Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Research Areas Neuroscience
Background The central nervous system octapeptide, neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance. Two G protein-coupled receptors, NPFF1 and NPFF2, have been isolated from human and rat central nervous system tissues. Localization of mRNA and binding sites of these receptors in the dorsal horn of the spinal cord, the lateral hypothalamus, the spinal trigeminal nuclei, and the thalamic nuclei suggest a role for NPFF in pain modulation.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution

ELISA: use at 1:1,000-1:5,000 dilution.
References & Data Sheet