Anti-NPFF2 Antibody

$374.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB,ELISA	Human

SKU: 56111 Categories: Antibody Products, Neuroscience and Signal Transduction Antibodies, Products

Overview

Product Name Anti-NPFF2 Antibody (56111)

Description Anti-NPFF2 Rabbit Polyclonal Antibody

Target NPFF2

Species Reactivity Human

Applications WB,ELISA

Host Rabbit

Clonality Polyclonal

Immunogen N-terminal sequence MNEKWDTNSSENWHPI and C-terminal sequence ELVMEELKETTNSSEI of human NPFF2.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS.

Buffer Formulation Phosphate Buffered Saline

Format Purified

Purification Purified by Protein A affinity chromatography

Specificity This antibody recognizes human NPFF2 (45 kD).

Target Name Neuropeptide FF receptor 2

Target ID NPFF2

Uniprot ID Q9Y5X5

Alternative Names G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, Neuropeptide G-protein coupled receptor

Gene Name NPFFR2

Sequence Location Cell membrane; Multi-pass membrane protein.

Biological Function Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Research Areas Neuroscience

Background The central nervous system octapeptide, neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance. Two G protein-coupled receptors, NPFF1 and NPFF2, have been isolated from human and rat central nervous system tissues. Localization of mRNA and binding sites of these receptors in the dorsal horn of the spinal cord, the lateral hypothalamus, the spinal trigeminal nuclei, and the thalamic nuclei suggest a role for NPFF in pain modulation.

Handling

Storage Store at 2 - 8ºC. Do not Freeze.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution

ELISA: use at 1:1,000-1:5,000 dilution.

Data Sheet