Nitrotyrosine Monoclonal Antibody
Catalog No.: 56456 (clone 39B6)
Format: 100ug in PBS pH 7.4, 50% glycerol, and 0.09% sodium azide. Purified by Protein G affinity chromatography.
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P- 450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, its plasma concentration may be a useful determinant of NO- dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.
Antigen: 3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA.
Accession no.: Not applicable
Host Species: Mouse
Antibody Class: IgG2a
Specificity: This antibody recognizes 3-nitrotyrosine moieties, species-independent. No detectable reactivity with non-nitrated tyrosine.
Immunoblotting: use at 1.0ug/ml.
Positive control: Linsidomine (SIN-1)-treated BSA.
Immunohistochemistry: use at 1-5ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.