Nitrotyrosine ELISA Kit (ECP7909)
$711.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Nitrotyrosine ELISA Kit (ECP7909)
Description Nitrotyrosine ELISA Kit (ECP7909) is a Competitive Inhibition ELISA for the quantitiation of Nitrotyrosine in a variety of aqueous sample types.
Target Nitrotyrosine
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 226.19 g·mol−1
Background Minimum Level of Detection: 1.35 ng/mL
Dynamic range of quantitation: 3.70-300 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 3.70-300 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Nitrotyrosine Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Nitrotyrosine Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Nitrotyrosine
Alternative Names SCHEMBL38174
PubChem ID 9834478
Research Areas Biochemicals
Background Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. Nitrotyrosine is identified as an indicator or marker of cell damage, inflammation as well as NO (nitric oxide) production.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.