Anti-NFκB, p50 Antibody
Catalog No.: 12508-25 25ug, 12508-100 100ug
Size: Rabbit IgG in borate-buffered saline, pH 8.2, purified by immunoaffinity chromatography.
Nuclear factor kappaB (NFkappaB) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of the kappa light chain gene in B lymphocytes. NFkappaB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkappaB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines, and viral infection, NFkappaB is phosphorylated and degraded by the proteosome. The active NFkappaB heterodimer is translocated into the nucleus and induces gene expression.
Immunogen: Synthetic peptide corresponding to aa 4-21 of mouse NFκB p50.
Host Species: Rabbit
Stabilizers: 50% glycerol
Preservatives: 0.09% sodium azide
This antibody recognizes mouse and rat NF- κB p50. Detects a band of ~50kDa in Western blot.
Immunoblotting: use at 0.5-1.0ug/ml.
Positive control: Rat spleen tissue lysate.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.