NFκB-p65 (Phospho-Thr254) Polyclonal Antibody
Catalog No.: 43010
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
NFκB is a transcription factor found in almost all cell types and is involved in many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFκB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. such as p65-p50 and p65-c-Rel complexes. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression.
Antigen: Peptide sequence that includes phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from human NFκB and conjugated to KLH.
Accession no.: Q04206, NP_001138610.1
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat NFκB only when phosphorylated at threonine 254.
Western blotting: use at dilution of 1:500- 1:1,000. A band of ~65kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term use.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.