Anti-Neurturin Antibody (1121)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
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Overview
Product Name Anti-Neurturin Antibody (1121)
Description Anti-Neurturin Rabbit Polyclonal Antibody
Target Neurturin
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 178-193 of human neurturin.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, neurturin (14 kD).
Target Name Neurturin
Target ID Neurturin
Uniprot ID Q99748
Gene Name NRTN
Gene ID 4902
Accession Number NP_004549
Sequence Location Secreted.
Biological Function Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
Research Areas Neuroscience
Background Glial cell line-derived neurotrophic factor (GDNF) plays a key role in the conrol of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. (NTN). Physiological responses to NTN require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAS encoding NTNRalpha from human, rat, chicken, and mouse have been cloned and referred to as GFRalpha-2, GDNFRbeta, Ret ligand 2 (RETL2), and TGF-beta-related neurotrophic factor receptor 2 (TmR2). NTN forms a complex with GFRalpha-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations.
Application Images
Description Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.
Description Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 ug/mL.
Description Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 ug/ml. Green: Neuriturin Antibody (1121)
Red: Phylloidin staining
Blue: DAPI staining
Description Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 ug/ml.
Description Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 ug/mL.
Description Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 ug/mL. Green: Neuriturin Antibody (1121)
Red: Phylloidin staining
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution.
References & Data Sheet