Anti-Myoferlin Antibody (90020)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC Human, Mouse
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SKU: 90020 Categories: , ,
Overview
Product Name Anti-Myoferlin Antibody (90020)
Description Anti-Myoferlin Mouse Monoclonal Antibody
Target Myoferlin
Species Reactivity Human, Mouse
Applications WB,ICC
Host Mouse
Clonality Monoclonal
Clone ID 7D2
Isotype IgG2a
Immunogen Synthetic peptide derived from the N-terminal domain of the human FER1L3 protein. Accession no. Q9NZM1.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse FER1L3.
Target Name Myoferlin
Target ID Myoferlin
Uniprot ID Q9NZM1
Alternative Names Fer-1-like protein 3
Gene Name MYOF
Sequence Location Cell membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein. Cytoplasmic vesicle membrane; Single-pass type II membrane protein. Note=Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.
Biological Function Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity). {ECO:0000250}.
Research Areas Cancer research
Background Ferlin proteins have been implicated in vesicle trafficking in a variety of physio- logical settings. All mammalian ferlins derive their names based on homology to the C. elegans protein FER-1 (FERtilization defective-1). FER1L3 (myoferlin, MYOF), has not yet been directly associated with a distinct mammalian disorder, but recent microarray and proteomic studies in the cancer literature have reported overexpression of MYOF in breast cancer specimens and relevant cell lines. Therefore, MYOF may be an important protein in breast cancer cells for their mobilization during cellular migration and/or invasion.
Application Images
Description Immunoblotting: use at 1-10ug/ml. A band of ~230kDa is detected. Detection of myoferlin in (1) C2C12 cell lysate and (2) human fibroblast cell lysate with #90020.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~230kDa is detected.

Immunocytochemistry: use at 1- 20ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet