MyoD (Phospho-Ser200) Polyclonal Antibody
Catalog No.: 43077
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
MyoD encodes a nuclear protein that is a member of the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest which is required for myogenic initiation. MyoD is also involved in muscle regeneration. It activates its own transcription which may stabilize the commitment to myogenesis.
Antigen: Peptide sequence that includes phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from human MyoD and conjugated to KLH.
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat MyoD only when phosphorylated at serine 200.
Accession no.: P15172, NP_002469.2
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~40kDa is detected.
These are recommended working dilutions. Endusers should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.