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Anti-Myc (phospho-Thr358) Antibody (43035)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IF Human
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SKU: 43035 Categories: ,
Overview
Product Name Anti-Myc (phospho-Thr358) Antibody (43035)
Description Anti-Myc (phospho-Thr358) Rabbit Polyclonal Antibody
Target Myc (phospho-Thr358)
Species Reactivity Human
Applications WB,IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from human Myc and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human Myc only when phosphorylated at threonine 358.
Target ID Myc (phospho-Thr358)
Post Translational Modification Myc (phospho-Thr358)
Target Phospho-thr358
Research Areas Phosphospecific Antibodies
Background Myc (c-Myc) is a regulator gene that codes for transcription factor Myc. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. A mutated version of Myc is found in many cancers which causes Myc to be constitutively expressed.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~60kDa is detected.

Immunohistochemistry: use at dilution of 1:50-1:100.

Immunofluorescence: use at 1:100-1:200.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet