Anti-MSH2 Antibody

$219.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
SKU: 34075 Categories: , ,
Overview
Product Name Anti-MSH2 Antibody (34075)
Description Anti-MSH2 Mouse Monoclonal Antibody
Target MSH2
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G410.1
Isotype IgG2b
Immunogen Recombinant human MSH2
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Human MSH2. Reactivity with other species has not been investigated.
Target Name DNA mismatch repair protein Msh2
Target ID MSH2
Uniprot ID P43246
Alternative Names hMSH2, MutS protein homolog 2
Gene Name MSH2
Sequence Location Nucleus, Chromosome
Biological Function Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha rPubMed:10078208, PubMed:10660545, PubMed:15064730, PubMed:17611581, PubMed:21120944, PubMed:26300262, PubMed:9564049, PubMed:9822679, PubMed:9822680}.
Research Areas Cancer research
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 6 for 10-30 minutes. Detection of MSH2 in human esophagus with #34075 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 6 for 10-30 minutes. STORAGE AND STABILITY
References & Data Sheet