Product Name Anti-Mouse Sperm Protein sp56 Antibody (55103)
Description Anti-Mouse Sperm Protein sp56 Mouse Monoclonal Antibody
Target Mouse Sperm Protein sp56
Species Reactivity Mouse, Hamster
Clone ID 4B2
Immunogen Mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Purification Purified by Protein G affinity chromatography
Specificity These antibodies specifically recognize mouse sperm protein sp56. They will react with native or denatured, non-reduced sp56 but not with denatured, reduced sp56. These antibodies do not cross-react with human sperm. Relative antibody titers for antibody binding to solid phase sp56: 55101 = 66pM, 55102 = 4nM, 55103 = 29nM, 55104 = 70nM
Target ID Mouse Sperm Protein sp56
Research Areas Reproductive Biology
Storage These antibodies are stable for at least on (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunohistochemistry on zinc-formaldehyde fixed, paraffin-embedded mouse testes to localize sp56 on mouse sperm and in immunoblots or ELISA to detect sp56.
References & Data Sheet
References Cheng A. et al. 1994. Sperm-Egg Recognition in the Mouse: Characterization of sp56, A Sperm Protein Having Specific Affinity for ZP3. J Cell Biol 125: 867-878.Bookbinder LH et al. 1995. Tissue- and Species-Specific Expression of sp56, a Mouse Sperm Fertilization Protein. Science 269: 86-89
Data Sheet Download PDF Data Sheet