Goat Anti-Mouse IgG2c Antibody, Alkaline Phosphatase Conjugated
Catalog No.: 83702
Format: 0.5mg/ml in HEPES, pH 7.1, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2, 0.2% BSA, 0.09% sodium azide, conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1.
Antigen: Mouse IgG2c
Host Species: Goat
Specificity: Antibody is affinity-purified on mouse IgG2c. By immunoblotting and ELISA, this antibody reacts specifically with IgG2c of C57Bl/6, SJL, C57BL/10, CB20, and C57BL/6 x Balb/c mice. Cross-reactivity with other mouse IgG subtypes is not detected.
Immunoblotting: use at a dilution of 1:1,000-1:10,000.
Immunohistochemistry: use at a dilution of 1:100-1:1,000.
Immunocytochemistry: use at a dilution of 1:100-1:1,000.
ELISA: use at a dilution of 1:1,000-1:10,000.
These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least 1 year at 2-8°C.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.