Mitosin Monoclonal Antibody
Catalog No.: 3320
Size: 100 ug or 200 ug Protein G- purified antibody in PBS, pH 7.4
Antigen: GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.
Host Species: Mouse
Antibody Class: IgG2a/κ
Preservatives: None. Available on request.
This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Immunoblotting: use at 1-10 ug/ml.
Immunoprecipitation: use at 1-10 ug/ml.
Immunohistochemistry: use at 10 ug/ml with frozen or paraffin-embedded sections.
Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.
Allred, DC et al. (1997) Lab Invest 15A (69);
Zhu, X et al. (1995), Mol Cell Biol 15: 5017-5029.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.