mGluR1/5 Glutamate Receptor Monoclonal Antibody
Catalog No.: 56545 (clone S75-3)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
The metabotropic glutamate receptors (mGluRs) are active through an indirect metabotropic process. They are members of the group C family of G-protein-coupled receptors, or GPCRs. Like all glutamate receptors, mGluRs bind glutamate which functions as an excitatory neurotransmitter. Metabotropic glutamate receptors are not ion channels. Instead, they activate biochemical cascades, leading to the modification of other proteins. This can lead to changes in the synapse’s excitability. Eight different types of mGluRs, designated mGluR1 to mGluR8, are divided into groups I, II, and III. Receptor types are grouped based on receptor structure and physiological activity.
Antigen: Fusion protein corresponding to aa 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession no. P31424).
Host Species: Mouse
Antibody Class: IgG2a
This antibody recognizes human, mouse, and rat mGluR1 and mGluR5.
Immunoblotting: use at 1ug/ml. A band of ~130kDa is detected. Smaller fragments due to proteolysis are detected as well.
Positive control: Rat brain lysate.
These are recommended concentrations. User should determine optimal concentrations for their application.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.