Anti-mGluR1/5 Glutamate Receptor Antibody (56545)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP Human, Mouse, Rat
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Overview
Product Name Anti-mGluR1/5 Glutamate Receptor Antibody (56545)
Description Anti-mGluR1/5 Glutamate Receptor Mouse Monoclonal Antibody
Target mGluR1/5 Glutamate Receptor
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,IP
Host Mouse
Clonality Monoclonal
Clone ID S75-3
Isotype IgG2a
Immunogen Fusion protein corresponding to aa 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession no. P31424).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat mGluR1 and mGluR5.
Target Name Metabotropic glutamate receptor 5
Target ID mGluR1/5 Glutamate Receptor
Uniprot ID P31424
Alternative Names mGluR5
Gene Name Grm5
Sequence Location Cell membrane, Multi-pass membrane protein
Biological Function G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium sPubMed:1320017, PubMed:21795692}.
Research Areas Neuroscience
Background The metabotropic glutamate receptors (mGluRs) are active through an indirect metabotropic process. They are members of the group C family of G-protein-coupled receptors, or GPCRs. Like all glutamate receptors, mGluRs bind glutamate which functions as an excitatory neurotransmitter. Metabotropic glutamate receptors are not ion channels. Instead, they activate biochemical cascades, leading to the modification of other proteins. This can lead to changes in the synapse's excitability. Eight different types of mGluRs, designated mGluR1 to mGluR8, are divided into groups I, II, and III. Receptor types are grouped based on receptor structure and physiological activity.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-3 (56545). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (56545) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (56545). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (56545) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) mGluR1/5 glutamate receptor Antibody. (D) Composite.
Description Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33 (56545). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody (56545) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~130kDa is detected. Smaller fragments due to proteolysis are detected as well.

Positive control: Rat brain lysate.

These are recommended concentrations. User should determine optimal concentrations for their application.
References & Data Sheet