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Anti-MEK2 (Phospho-Thr394) Antibody (43008)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human
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Overview
Product Name Anti-MEK2 (Phospho-Thr394) Antibody (43008)
Description Anti-MEK2 (Phospho-Thr394) Rabbit Polyclonal Antibody
Target MEK2 (Phospho-Thr394)
Species Reactivity Human
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of threonine (P-G-T(p)-P-T) derived from human MEK2 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human MEK2 only when phosphorylated at threonine 394.
Target Name Dual specificity mitogen-activated protein kinase kinase 2
Target ID MEK2 (Phospho-Thr394)
Uniprot ID P36507
Gene Name MAP2K2
Gene ID 601263
Post Translational Modification MEK2 (Phospho-Thr394)
Target Phospho-thr394
Sequence Location Cytoplasm, Membrane
Biological Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126). {UniProtKB:Q63932, PubMed:29433126}.
Research Areas Phosphospecific Antibodies
Background MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase plays an important role in mitogen growth factor signal transduction. It phosphorylates and activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Western blotting: use at dilution of 1:500- 1:1,000. A band of ~44kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet