MEF2a (Phospho-Thr312) Polyclonal Antibody
Catalog No.: 43039
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression including the DNA binding regulatory protein myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many muscle-specific genes. The MEF2 genes are members of the MADS gene family that also includes several homeotic genes and other transcription factors all of which share a conserved DNA-binding domain.
Antigen: Peptide sequence that includes phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from human MEF2a and conjugated to KLH.
Accession no.: Q02078, NP_001124398.1
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat MEF2a only when phosphorylated at threonine 312.
Immunohistochemistry: use at dilution of 1:50- 1:100.
Immunofluorescence: use at dilution of 1:100- 1:200.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term use.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.