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Anti-Measles Phosphoprotein Antibody (90019)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC,ELISA Measles Virus
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SKU: 90019 Categories: , ,
Overview
Product Name Anti-Measles Phosphoprotein Antibody (90019)
Description Anti-Measles Phosphoprotein Mouse Monoclonal Antibody
Target Measles Phosphoprotein
Species Reactivity Measles Virus
Applications WB,ICC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 9H4
Isotype IgG1
Immunogen Recombinant Measles virus phosphoprotein.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with Measles virus phosphoprotein.
Target Name Phosphoprotein
Target ID Measles Phosphoprotein
Uniprot ID P35974
Alternative Names Protein P
Gene Name P/V
Biological Function Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.
Research Areas Infectious Disease
Background Measles virus (MeV) is an enveloped RNA virus in the Morbillivirus genus of the Paramyxoviridae family. Its non- segmented, negative-sense, single- stranded RNA genome is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid. Transcription and replication are carried out on this N:RNA complex by the viral polymerase complex which consists of two components, the large protein (L) and the phosphoprotein (P). MeV N consists of two regions: a structured N-terminal moiety, NCORE (aa 1–400), which contains all the regions necessary for self-assembly and RNA-binding, and a C-terminal domain, NTAIL(aa 401–525) that is intrinsically unstructured and is exposed at the surface of the viral nucleocapsid.
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions ELISA: use at 0.1-1.0ug/ml

Immunoblotting:

User should use at 1-5ug/ml. A band of ~70kDa is detected

Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet