Leucine-Rich Repeat Kinase 2 (LRRK2) Monoclonal Antibody
Catalog no.: 56582 (clone S231B-34)
Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is an enzyme that in humans is encoded by the PARK8 gene. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson’s disease type 8 and Crohn’s disease which suggests that these two diseases share a common pathway. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons.
Antigen: Fusion protein corresponding to aa 841-960 of human LRRK2. This sequence is 81% identical in mouse, 80% identical in rat, and <30% identical in LRRK1. Accession nos: NP_940980, Q5S007 Gene ID: 120892 Host Species: Mouse Antibody Class: IgG2a Specificity: This antibody recognizes human and rat LRRK2.
Immunoblotting: use at 1-5ug/ml. A band of >200kDa is detected.
Immunofluorescence: use at 10ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their application.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.